Immunological laboratory

The Laboratory of the Centre was organized one of the first in Astana in 1997. We are among the first to introduce the PCR method (polymerase chain reaction). A real-time examination is conducted for all sexually transmitted infections and other infectious diseases at the genetic level. There is a permission from the regime commission for carrying out diagnostic studies with microorganisms of the 3rd and 4th groups of pathogenicity.

Within the framework of the Project "Transfer of Technologies and Institutional Reforms in the Laboratory Service Sector", the laboratories of the Centre for Dermatology and Sexually Transmitted Diseases were evaluated by CDC experts and local laboratory service consultants on a standardized basis using the F-LAT tool developed by the World Health Organization and adapted by CDC and IQLS in the laboratories of the Sanitary - Epidemiological Surveillance Medical Center of Kazakhstan.

The laboratory conducts the following types of research:

  • PCR (polymerase chain reaction) on Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Gardnerella vaginalis, Trichomonas vaginalis, Herpes sympl.Virus II-I, Cytomeqalovirus, Neisseria qonorrhoeae, Candida albicans, HPV (16-18) types;
  • RIF (immunofluorescence reaction) on Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Gardnerella vaginalis, Trichomonas vaginalis;
  • ELISA (enzyme immunoassay) on Chlamydia trachomatis (Ig M; IgG;IgA;), Ureaplasma urealyticum (IgG, IgА), Mycoplasma hominis (IgG, IgА), Trichomonas vaginalis (IgG), , Herpes sympl.VirusІІ-І (Ig M; IgG), Cytomeqalovirus (Ig M; IgG), Candida albicans (IgG), Rubella (Ig M; IgG), Toxoplasma qondii (Ig M; IgG), Treponema pallidum (Ig M; IgG), as well as parasitic diseases of opisthorchiasis, giardiasis;
  • ELISA (enzyme immunoassay) on thyroid hormones (TSH, T3, T4, T4-free, anti-thyroglobulin autoantibodies, antibodies to thyroid peroxidase);
  • ELISA (enzyme immunoassay) on sex hormones (LH, FSH, prolactin, progesterone, estradiol, cortisol, testosterone);
  • ELISA (enzyme immunoassay) on viral hepatitis;
  • Allergodiagnostics by ELISA.

The laboratory is equipped with the modern, high-tech equipment. The advantage of this equipment is its high specificity, sensitivity, versatility and automation of the results recording, which gives the rapidity and quality of getting the research results:

  • PCR amplifiers “Bio-Rad iQ 5” and “Rotor-Gene Q” in real time;
  • There is a fluorescent microscope “Leica DM-1000” for the RIF;
  • For the enzyme immunoassay there is an automated ELISA analyzer Evolis, a microplate reader Bio-Rad-680, an EL-800 analyzer in the laboratory;
  • Thermocycler of nucleic acids by PCR method C 1000 with optical module CFX96 (Bio-Rad)

Head of Immunological laboratory – Sadykova Gulnar Dusenbayevna.

The staff of the laboratory also includes: 2 laboratory assistants, 5 laboratory technicians of the highest, second and first qualification categories.

The employees of the laboratory are qualified and have a great practical experience of work, participate in scientific and practical conferences, seminars held in the Republic of Kazakhstan and in the near abroad. The employees underwent further training in the specialty “laboratory work” in Russian Medical Academy of Continuing Professional Education in Moscow and St. Petersburg.

Participation in the Program of External Quality Assessment (PEQA) of Vector-Best CJSC, dedicated to the problems of quality control of laboratory investigations in the field of immunological analysis in the section of hepatitis B (HBsAg).

Glossary

What is PCR?

  • PCR (polymerase chain reaction) is a molecular genetic method of investigation. By the method of PCR, it is possible to determine the pathogen directly in the clinical material (scraping of epithelial cells from the cervical canal, urethra, conjunctiva of the eyes, posterior pharyngeal wall, urine sediment, sperm, etc.). The method allows to diagnose not only acute, but latent forms of infections.
  • PCR is effective in the diagnosis of difficult to cultivate, uncultivated and persistent pathogenic microorganisms.

What is ELISA (EIA)?

  • ELISA (Immunoenzymatic analysis) is a reaction of the interaction of specific antibodies to the pathogen’s antigen. ELISA is widely used in medicine to diagnose infectious diseases, autoimmune diseases, intrauterine infections. ELISA has the advantage in comparison with other methods of high specificity, sensitivity, ease of reaction, the detection of antibodies to immunoglobulins of various classes, the determination of antibody titer.

What is RIF?

  • RIF (the reaction of immunofluorescence) is the reaction of detection of antigens in the treatment of the material with specific monoclonal antibodies. The RIF is widely used for the diagnosis of urogenital chlamydiosis, ureaplasmosis, mycoplasmosis, gardnerelleza, herpes simplex virus, human papilloma virus, etc. Sensitivity and specificity of the method is 65-95%, which depends on the qualification of the specialist. The method is based on the observation of microscopic objects using their ability to glow.

The Laboratory of the Centre was organized one of the first in Astana in 1997. We are among the first to introduce the PCR method (polymerase chain reaction). A real-time examination is conducted for all sexually transmitted infections and other infectious diseases at the genetic level. There is a permission from the regime commission for carrying out diagnostic studies with microorganisms of the 3rd and 4th groups of pathogenicity.

Within the framework of the Project "Transfer of Technologies and Institutional Reforms in the Laboratory Service Sector", the laboratories of the Centre for Dermatology and Sexually Transmitted Diseases were evaluated by CDC experts and local laboratory service consultants on a standardized basis using the F-LAT tool developed by the World Health Organization and adapted by CDC and IQLS in the laboratories of the Sanitary - Epidemiological Surveillance Medical Center of Kazakhstan.

The laboratory conducts the following types of research:

  • PCR (polymerase chain reaction) on Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Gardnerella vaginalis, Trichomonas vaginalis, Herpes sympl.Virus II-I, Cytomeqalovirus, Neisseria qonorrhoeae, Candida albicans, HPV (16-18) types;
  • RIF (immunofluorescence reaction) on Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Gardnerella vaginalis, Trichomonas vaginalis;
  • ELISA (enzyme immunoassay) on Chlamydia trachomatis (Ig M; IgG;IgA;), Ureaplasma urealyticum (IgG, IgА), Mycoplasma hominis (IgG, IgА), Trichomonas vaginalis (IgG), , Herpes sympl.VirusІІ-І (Ig M; IgG), Cytomeqalovirus (Ig M; IgG), Candida albicans (IgG), Rubella (Ig M; IgG), Toxoplasma qondii (Ig M; IgG), Treponema pallidum (Ig M; IgG), as well as parasitic diseases of opisthorchiasis, giardiasis;
  • ELISA (enzyme immunoassay) on thyroid hormones (TSH, T3, T4, T4-free, anti-thyroglobulin autoantibodies, antibodies to thyroid peroxidase);
  • ELISA (enzyme immunoassay) on sex hormones (LH, FSH, prolactin, progesterone, estradiol, cortisol, testosterone);
  • ELISA (enzyme immunoassay) on viral hepatitis;
  • Allergodiagnostics by ELISA.

The laboratory is equipped with the modern, high-tech equipment. The advantage of this equipment is its high specificity, sensitivity, versatility and automation of the results recording, which gives the rapidity and quality of getting the research results:

  • PCR amplifiers “Bio-Rad iQ 5” and “Rotor-Gene Q” in real time;
  • There is a fluorescent microscope “Leica DM-1000” for the RIF;
  • For the enzyme immunoassay there is an automated ELISA analyzer Evolis, a microplate reader Bio-Rad-680, an EL-800 analyzer in the laboratory;
  • Thermocycler of nucleic acids by PCR method C 1000 with optical module CFX96 (Bio-Rad)

Head of Immunological laboratory – Sadykova Gulnar Dusenbayevna.

The staff of the laboratory also includes: 2 laboratory assistants, 5 laboratory technicians of the highest, second and first qualification categories.

The employees of the laboratory are qualified and have a great practical experience of work, participate in scientific and practical conferences, seminars held in the Republic of Kazakhstan and in the near abroad. The employees underwent further training in the specialty “laboratory work” in Russian Medical Academy of Continuing Professional Education in Moscow and St. Petersburg.

Participation in the Program of External Quality Assessment (PEQA) of Vector-Best CJSC, dedicated to the problems of quality control of laboratory investigations in the field of immunological analysis in the section of hepatitis B (HBsAg).

Glossary

What is PCR?

  • PCR (polymerase chain reaction) is a molecular genetic method of investigation. By the method of PCR, it is possible to determine the pathogen directly in the clinical material (scraping of epithelial cells from the cervical canal, urethra, conjunctiva of the eyes, posterior pharyngeal wall, urine sediment, sperm, etc.). The method allows to diagnose not only acute, but latent forms of infections.
  • PCR is effective in the diagnosis of difficult to cultivate, uncultivated and persistent pathogenic microorganisms.

What is ELISA (EIA)?

  • ELISA (Immunoenzymatic analysis) is a reaction of the interaction of specific antibodies to the pathogen’s antigen. ELISA is widely used in medicine to diagnose infectious diseases, autoimmune diseases, intrauterine infections. ELISA has the advantage in comparison with other methods of high specificity, sensitivity, ease of reaction, the detection of antibodies to immunoglobulins of various classes, the determination of antibody titer.

What is RIF?

  • RIF (the reaction of immunofluorescence) is the reaction of detection of antigens in the treatment of the material with specific monoclonal antibodies. The RIF is widely used for the diagnosis of urogenital chlamydiosis, ureaplasmosis, mycoplasmosis, gardnerelleza, herpes simplex virus, human papilloma virus, etc. Sensitivity and specificity of the method is 65-95%, which depends on the qualification of the specialist. The method is based on the observation of microscopic objects using their ability to glow.